Staff – University of Copenhagen

Forward this page to a friend Resize Print Bookmark and Share

GeoGenetics > Staff

Deep sequencing of RNA from ancient maize kernels

Research output: Research - peer-reviewJournal article

Documents

Sarah Louise Fordyce, Maria del Carmen Avila Arcos, Morten Rasmussen, Enrico Cappellini, J. Alberto Romero-Navarro, Nathan Wales, David Eugenio Alquezar Planas, Steven Penfield, Terence A. Brown, Jean-Philippe Vielle-Calzada, Rafael Montiel, Tina Jørgensen, Nancy Odegaard, Michael Jacobs, Bernardo Arriaza, Thomas F. G. Higham, Christopher Bronk Ramsey, Eske Willerslev, Tom Gilbert

The characterization of biomolecules from ancient samples can shed otherwise unobtainable insights into the past. Despite the fundamental role of transcriptomal change in evolution, the potential of ancient RNA remains unexploited - perhaps due to dogma associated with the fragility of RNA. We hypothesize that seeds offer a plausible refuge for long-term RNA survival, due to the fundamental role of RNA during seed germination. Using RNA-Seq on cDNA synthesized from nucleic acid extracts, we validate this hypothesis through demonstration of partial transcriptomal recovery from two sources of ancient maize kernels. The results suggest that ancient seed transcriptomics may offer a powerful new tool with which to study plant domestication.
Original languageEnglish
Article numbere50961
JournalPLoS ONE
Volume8
Issue number1
Number of pages9
ISSN1932-6203
DOIs
StatePublished - 11 Jan 2013

Number of downloads are based on statistics from Google Scholar and www.ku.dk


No data available

ID: 46089758